Sebastien Levesque, BSc, MSc

Visiting PhD Candidate

I grew up in New Brunswick and I started studying biotechnology in 2012. Since then, I have been studying and teaching biotechnology and microbiology in New Brunswick, Ontario, Quebec, and even France. I completed my BSc in biotechnology at Collège la Cité (Ottawa, ON) in 2016 and I moved to Quebec City to undertake a MSc in microbiology at Université Laval under the supervision of Prof. Sylvain Moineau, internationally renowned for his pioneer work in the CRISPR field. After completing my MSc in 2018, I started my PhD in molecular medicine at the CHU de Québec Research Center – Université Laval under the supervision of Prof. Yannick Doyon, an expert in genome editing whose research is focused on the therapeutic applications of CRISPR-Cas9. I have been working in collaboration with Prof. Julian J. Lum lab since the beginning of my PhD and I am now a visiting student in beautiful British Columbia. During my lab exchange, I will take advantage of the expertise of Prof. Lum to learn many things about metabolism, autophagy, immunotherapy, and CAR T cell engineering.

RESEARCH FOCUS

My research focuses on the development of strategies to efficiently and precisely engineered T cells with CRISPR-Cas9 for cancer immunotherapy. I am interested in how human cells repair Cas9-mediated DNA double-strand breaks and how we can take advantage of these DNA repair pathways to introduce precise genome modifications.

PUBLICATIONS

Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest J-F, Levesque S, Loehr J, Mouchiroud M, Cyr D, Water PJ, Laplante M, Moineau S, Goulet A, Doyon Y. 2020. Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Research 30:107-117

 

Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A. 2019. Cas9 allosteric inhibition by the Anti-CRISPR protein AcrIIA6. Molecular Cell 76:922-937.e7